Exportation of Mouse Vas Deferens Protein, a Protein Without a Signal Peptide, from Mouse Vas Deferens Epithelium: A Model of Apocrine Secretion

Proteomic analysis of androgen-regulated protein expression in a mouse fetal vas deferens cell line.

During sex differentiation, androgens are essential for development of the male genital tract. The Wolffian duct is an androgen-sensitive target tissue that develops into the epididymis, vas deferens, and seminal vesicle. The present study aimed to identify androgen-regulated proteins that are involved in development of Wolffian duct-derived structures. We have used male mouse embryos transgeni...

Obstruction of the vas deferens alters protein secretion by the rat caput epididymidal epithelium in vivo.

Obstruction of epididymal lumen fluid flow alters the intraluminal environment and potentially changes epididymal epithelial cell function when those functions are dependent on intraluminal regulatory molecules. This investigation tested the hypothesis that obstruction of the rat vas deferens alters caput epididymidal protein synthesis and secretion In vivo. Adult male rats were subjected to va...

Vas deferens--a model used to establish sympathetic cotransmission.

The vas deferens has been used as a model for many diverse studies of different aspects of autonomic neurotransmission since its introduction including, in particular, sympathetic cotransmission involving release of ATP and neuropeptide Y together with noradrenaline and prejunctional and postjunctional neuromodulation. It has also been used to study sympathetic reinnervation following vasectomy...

EXPRESSION OF CFTR mRNA IN NASAL EPITHELIUM AND VAS DEFERENS

Neurotransmitter-stimulated ion transport by cultured porcine vas deferens epithelium.

A collagenase-based dissociation technique has been developed to routinely establish monolayer cultures of freshly isolated porcine vas deferens epithelium. Cells isolated from each tissue are transferred to 25-cm(2) tissue culture flasks and grown in a standard cell culture medium. Flasks reach confluency in 3-4 days, and cells are subsequently seeded onto permeable supports. Cultured cells di...